Monday, February 24, 2020

Societal marketing concept Essay Example | Topics and Well Written Essays - 1000 words

Societal marketing concept - Essay Example This might also help in enhancement of the total sale and profitability of the organization in the entire market as compared to others. Hence, it might be depicted from the above mentioned points that customers act as the focal point of any organization operating in any segment. Thus, the motive of achieving organizational goals and profits might be attained mainly through customer satisfaction and loyalty as compared to other aspects. Characteristics of marketing concept According to Kotler & Armstrong (2010) the concept of marketing is changing rapidly in this age of globalization and industrialization. In this age, marketing concept is entirely dependent over customer values and satisfaction that might be witnessed in four different stages such as production concept, product concept, selling concept and societal marketing concept. Production concept According to the strategies of previous era, the demand of the product is entirely dependent over its features and price. This means that, if the underlining features of the product are praiseworthy or satisfactory then it would surely be liked by the customers of varied age groups and income groups. However, in today’s age, the concept of production has entirely changed. Now, the demand of any specific product might be retained in the market only by presenting the desired features within it. So that, the target customers of the product might get satisfied entirely thereby amplifying the efficiency and productivity of the organization to a certain extent (Kotler & Armstrong, 2010). Along with this, good customer service is also another important aspect that might act as a backbone in enhancing the reliability and loyalty of the product among other substitutes. For example: the management of Macdonald’s always tries to present varied types of nutrients such as cheese, chilies, nuts and many others so as to satisfy the changing demands of the customers. As well as the management also desires to presen t warm greetings to its customers so as to retain them for longer period of time. Only then, the popularity and demand of the products and the organization might remain dominant in the market among other and the rate of switch over costs of the customers towards other brands (Burger King) might be reduced significantly in this aggressive market among others. Thus it might be stated that relationship marketing and customer oriented products acts as the prime essence of today’s publicity concept. Product Concept In order to retain sustainability and competitive advantage, most of the organizations in this era desire to present value-added products at a lowest price. This strategy acts as a stimulating factor thereby enhancing the demand of the products of the organization such as HP as compared to others. Along with this, such a competitive strategy might also prove effective in satisfying the demands and needs of the customers thereby amplifying their level of loyalty (Tiddy & Bessant, 2010). Apart from this, the organization of HP also desires to implement the strategy of presenting qualitative products so as to retain its dominance and fame in the segment of electronics among many other existing rivals (Porter, 1986). Selling Concept In this age of stiff economy and tough competition, the insurance organizations such ICICI Prudential, Kotak Mahindra are striving hard to create a strong foothold in this market. However, this might be possible only by enhancing the rate of satisfaction

Saturday, February 8, 2020

BIOTECHNOLOGY & GENETIC ANALYSIS Essay Example | Topics and Well Written Essays - 750 words

BIOTECHNOLOGY & GENETIC ANALYSIS - Essay Example This is mainly attributed to accumulation of pollutant along the path of river and hence bacterial population need to evolved catabolic capability to survive and hence more plasmid bearing bacterial population were found in second sample. Similarly most of the plasmid was found to be in size range more than 35 KB clearly indicates most of them are Conjugative plasmids as this group of plasmid has more number of genes compared to non-conjugative plasmid to carryout conjugation process and hence larger the size. Here re-suspension solution constitutes of glucose, EDTA and Tris each have its own role. Glucose provides osmotic stress and EDTA as chelating agents which binds to heavy metals and helps in disintegration of cell wall, Tris act as buffering agent and maintains pH of over all reactions to avoid any pH dependent side reaction. In this stage cell become highly fragile and some are break open. This solution is mixture of SDS and NaOH. Here NaOH provides alkaline condition which helps in cell lyses and denaturation of DNA while SDS dissolves cell wall constituents and induces extensive cell lyses. It also helps in proteins denaturation and precipitation. In this stage most of cell constituents get denatured including genomic DNA, But as plasmid is in its CCC (covalently closed circular) forms will not denatured completely and most of them remains in its native configuration. Step 4: Neutralisation Solution Here potassium acetate and acetic acid act as neutralizing agent to bring back the pH to normal. Similarly it induces the renaturation of DNA. Because of larger size most of the Genomic DNA remains denatured and mingled with proteins remains with cell debris while plasmid being smaller molecule except out to supernatant . Step 5: centrifugation at high speed; During this stage all cell derbies along with genomic DNA settled at the bottom of tube and being smaller in size plasmid remains in supernatant. Which subsequently used for further purification and transformation. Ans 3 protocol 6: Here we have two different observation 1) colonies from tube 2 grown as blue colored colonies 2) while from tube 3 there is mixture of blue and white. This can be explained as follows. In case of tube 2 there is only vector pGEM3Z used for transformation. The plasmid pGEM3Z have lacZ gene as marker which code for enzyme called beta glycosidase. After transformation cells where plated on LA supplemented with X-gal and IPTG. Now in presence of IPTG expression of lac Z induces and leads to synthesis of beta-glycosidase which subsequently acts on X-gal and cleaved it to chromogenic intermediate give rise to blue color. While in case of tube 3 there was plasmid vector along with insert gene (ligation mixture) and plated on similar plate after transformation. As vector pGEM3Z having MCS (multiple cloning sites) in side the lacZ gene any insertion or recombination leads to inactivation of lacZ (insertional inactivation). Inactive lacZ will not code for functional beta glycosidase and hence colonies having insertion give rise to white colors. In another scenario where cut plasmid re-ligated without any insertion during